外源Wnt3a对体外培养奶牛乳腺上皮细胞数量及Frizzled 1受体和β-catenin表达的影响

doi:10.11843/j.issn.0366-6964.2014.08.011

Abstract

Abstract:

This experiment was aimed at studying the effects of exogenous Wnt3a on bovine mammary epithelial cell (BMEC) number and the expression of Frizzled 1 receptor and β-catenin.BMEC was cultured by adherent tissue culture.β-casein of culture medium was detected by Western-blot.BMEC was cultured in medium with 0，10，25 and 50 ng•mL^-1 Wnt3a separately，and then the BMEC and culture medium at 10 days were collected.The expression of Frizzled 1 receptor and β-catenin mRNA was checked by real time RT-PCR.The results showed that cell numbers in 50 ng•mL^-1 Wnt3a group were significantly higher than those of control group.The expression of Frizzled 1 receptor significantly increased，and there was not much difference in β-catenin expression between control group and Wnt3a group.It was concluded that Wnt3a exerts effects on the expression of Frizzled 1 receptor，moreover，promotes the number of BMEC.Current evidence suggested that Wnt signal played roles in the regulation of mammary development.

CLC Number:

S852.16

CUI Ya-li，SUN Hai-yun，LI Jian-guo，GAO Yan-xia，ZHAO Yan-jiao，LI San-xia. Effect of Exogenous Wnt3a on Mammary Cell Number in Cow and the Expression of Frizzled 1 Receptor and β-catenin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1282-1287.

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Effect of Exogenous Wnt3a on Mammary Cell Number in Cow and the Expression of Frizzled 1 Receptor and β-catenin

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